|Other Names||Lady Ascot|
|Other|| Henry Ledore|
Mrs. Ascot is the mother of Randall Ascot who appeared in the game Professor Layton and the Miracle Mask. She had lived in the Ascot House in Stansbury with Randall and her husband 18 years prior to the game.
Mrs. Ascot wears a red dress, baby pink shoes and black, thick-framed glasses, similar to Randall's. She has grey hair tied up in a bun.
Chapter 7: The Reunion Inn
Professor Layton and the group are searching around Henry's Study for clues to their investigation, when Mrs. Ascot suddenly appears. They instantly recognize each other, and she asks what happened to Layton's hair. He introduces her to Emmy and Luke, and asks her if she was visiting Henry and Angela. She told him this was incorrect, however, and begins to tell him that after Randall has disappeared, she and her husband did what they could to find him. They hired rescue teams, in turn spending their entire fortune. It pained them to be in that house without Randall, so they moved to Monte d'Or with Henry. When her husband had passed away, she had nowhere to go, and so Henry offered for her to stay with him and Angela. He had settled her debts and prepared for Randall's return. When Layton comments on how Henry never discussed this with him, she tells him that he was quiet and didn't want to blow his own trumpet. She asks him to be kind to Henry, as he never fully accepted Randall's death, and thinks that if the Masked Gentleman knew Henry, he wouldn't be terrorizing the town. She wished Randall was still alive, as the two of them were like brothers.
At this point, Angela walks in, and upon seeing they were still conversing, she apologizes for interrupting. Mrs. Ascot asks if it was time for lunch, and Angela tells her she wanted to call her out before the food went cold. Angela once again apologizes for interrupting, and offered to wait till they were finished speaking, however Layton was done with their business there. Angela tells him they could wait for Henry to return as he wouldn't take long, but Layton had other things he wanted to attend to. She wishes them luck on finding the Mask of Order, as she believes it was the only way to save the city.
Epilogue: The City of Miracles
As Professor Layton is explaining everything to Randall, Sheffield brings out Mrs. Ascot to see him, and he is glad to see her again. She explains to him that Henry had taken such good care of her, she wouldn't know what to do without him.
"Randall's elusive mother had been kept well-hidden by Henry, waiting for the day her son would return. Layton deduced that she was the guarantor who gave Henry the rights to the land. Her needlework is top-notch and both Henry and Angela's clothes are her handiwork."