The Old Woman portrayed in the film looks a lot like Florence (Professor Layton and the Unwound Future). She has a purple hat with a teal flower on the right, a pink cardigan, a white shirt, a purple neck tie, a blue skirt, purple tights and pink shoes. She has a long, pointed nose and grey, curly hair. She also carries around a yellow parasol, which she uses to fly across the London bridge.
During the start of the film, Professor Layton is seen on the Tower Bridge with the Scotland Yard force to find out who or what silenced Big Ben. Professor Layton points to the Old Woman and says that it was her who silenced the bell. The Old Woman then reveals herself to be Don Paolo, who takes off the mask and attempts to make a getaway off the Tower Bridge with his yellow parasol.
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